Analysis And Maintenance Methods Of HPLC Columns

Flow Process of HPLC column
First, the solvent in the storage bottle is pumped into the chromatographic system by pump and then output, and then introduced into the sampler after flow and pressure measurement.

Second, object to be tested is injected by the sampler, passes through the chromatographic column with the mobile phase, and enters the detector after separation on the column, the detection signal is collected and processed by the data processing equipment, and the chromatogram is recorded.

Third, waste liquid flows into waste liquid bottle. Gradient elution can also be performed by a gradient controller when complex mixture separation is encountered. This is similar to the temperature rise of the gas chromatography procedure, except that the GC changes the temperature, while the HPLC changes the mobile phase polarity, allowing the sample components to be separated under optimum conditions.

Reversed phase chromatography is the most widely used technology in HPLC, mainly because it is suitable for the analysis of a large number of non-polar substances and many ionizable and ionic compounds. Most stationary phases used in reversed phase chromatography are natural hydrophobic substances. Therefore, the analytes are separated according to their hydrophobic interaction with stationary phases, and the hydrophobic mechanism can also be separated in the same way

Analysis And Maintenance Methods Of HPLC Columns