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Keratinase |
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Name |
Keratinase |
Product Series Name |
NutriGen Keratinase |
|
Activity |
100000-200000 U/g |
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|
Strain |
Parengyodontium album |
EC code |
EC 3.4.99.11 |
|
Form |
Powder,Liquid |
Carrier |
Starch |
|
Color |
White, Light yellow |
Loss on drying |
≤ 10% |
|
Working pH |
6.0-12.5 |
Optimum pH |
11 |
|
Working T |
30℃-70℃ |
Optimum T |
60℃ |
|
Particle size |
80% pass 40 mesh |
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|
Package |
25kg /bag |
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|
Storage |
Stored 6 months in dark, cool and dry conditions |
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Caution |
Avoid direct sunlight, high temperature |
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Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme china which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.
Recommended dosage is 50-100g/t with 50000 U/g in complete feed.
Functions of Keratinase
1. Supplement the deficiency of endogenous protease for animal.
2. Increase the digestibility of protein and amino acid in poultry and swine feed ingredients.
3. Degrade the trysin inhibitor and lectin, this is what endogenous protease can't do.
4. Optimized the use of lower digestibility feed ingredient e.g corn, sorghum, feather meal,soybean meal and blood plasma powder.
5. Creat a better environment for animal and decrease pollution from nitrogen for human.
Application of Keratinase
1. The matrix value of keratinase (100g/t)
|
Nutrient |
Poultry |
|
|
Value |
Amount provided in the diet |
|
|
Metabolizable energy（Mcal/kg） |
600 |
0.060 |
|
Crude protein, % |
5400 |
0.540 |
|
Lysine, % |
150 |
0.015 |
|
Methionine, % |
180 |
0.018 |
|
Cystine, % |
150 |
0.015 |
|
Threonine, % |
210 |
0.021 |
|
Tryptophane, % |
90 |
0.009 |
|
Isoleucine , % |
150 |
0.015 |
|
Arginine, % |
285 |
0.0285 |
|
Valine, % |
255 |
0.0255 |
2. Application case: Improve the performance and digestibility of nutrients.
Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.
Experimental design:
Table1. Experimental design
|
Item |
Diet |
|
PC |
Basal diet |
|
NC |
Basal diet-0.5% crude protein |
|
Group I |
NC+125g/t competitor |
|
Group II |
NC+100g/t Keratinase(30000U/g) |
Experimental cycle: 42 days
Results:
Table2 Effect of each treatment on the growth performance of AA broilers (g, %)
|
Items |
PC |
NC |
Group I |
Group II |
|
Initial weight,g |
62.79±0.31 |
62.58±0.29 |
62.56±0.33 |
62.33±0.27 |
|
1-21days |
||||
|
21stAG,g |
986.16ab±10.08 |
977.41b±8.22 |
1013.59ab±25.72 |
997.17ab±10.53 |
|
ADFI,g |
62.08± 1.43 |
60.93±1.10 |
62.09±2.04 |
58.71±2.12 |
|
ADG,g |
43.94±0.47 |
43.45±0.39 |
45.12±1.29 |
44.33±0.53 |
|
F/G |
1.41±0.03 |
1.40±0.03 |
1.38±0.05 |
1.33±0.04 |
|
Death rate,% |
1.43±1.57 |
3.45±2.21 |
5.38±6.24 |
3.33±5.24 |
|
22-42days |
||||
|
22ndAG,g |
1140.08±1.30 |
1135.33±5.97 |
1136.73±7.06 |
1138.33±5.41 |
|
42ndAG,g |
2985.77±41.56 |
2971.06±47.61 |
3058.21±58.29 |
3010.25±44.17 |
|
ADFI,g |
179.00±3.12 |
178.64±2.86 |
175.81±3.64 |
174.22±2.64 |
|
ADG,g |
87.88±2.01 |
87.42±2.43 |
91.50±2.85 |
89.14±2.20 |
|
F/G |
2.04±0.07 |
2.05±0.03 |
1.93±0.05 |
1.96±0.06 |
|
Death rate,% |
2.00±2.19 |
1.33±2.07 |
0.67±1.63 |
1.33±2.07 |
|
1-42days |
||||
|
ADFI,g |
120.51±2.39 |
119.78±1.63 |
118.94±2.01 |
116.47±1.60 |
|
ADG,g |
69.59±0.98 |
69.25±1.13 |
71.32±1.39 |
70.18±1.05 |
|
F/G |
1.73±0.02 |
1.73±0.02 |
1.67±0.02 |
1.66±0.03 |
|
Death rate,% |
3.43±3.76 |
4.78±1.73 |
6.05±5.74 |
4.67±4.59 |
Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).</span>
Diagram1 Effect of keratinase on the nutrient digestibility of broilers
Table3 Effect of keratinase on the amino acid digestibility of broilers （%）
|
Items |
PC |
NC |
Group I |
Group II |
|
Asp |
55.87±4.44 |
52.59±2.57 |
54.52±8.03 |
57.52±5.76 |
|
Thr |
63.09±7.47 |
61.47±5.32 |
62.17±5.74 |
63.69±5.19 |
|
Ser |
69.11±5.51 |
65.44±4.61 |
65.80±7.04 |
68.04±4.10 |
|
Glu |
69.31±3.41ab |
64.68±1.35b |
67.12±5.82 |
74.82±3.39a |
|
Gly |
55.36±15.14ab |
42.03±4.21b |
49.11±11.59b |
67.00±1.89a |
|
Ala |
31.82±2.11b |
34.51±4.37b |
35.35±5.19 |
35.92±4.82b |
|
Val |
46.32±0.75 |
45.59±1.66 |
44.68±4.68 |
50.18±3.73 |
|
Met |
72.53±1.35 |
70.69±2.65 |
75.17±3.79 |
74.29±3.31 |
|
Ile |
46.45±0.53b |
47.40±2.87ab |
47.47±5.60 |
51.65±4.65ab |
|
Leu |
56.28±1.57 |
52.87±7.19 |
55.44±8.01 |
53.05±4.90 |
|
Tyr |
50.93±3.67 |
47.91±4.34 |
50.94±10.97 |
56.55±8.06 |
|
Phe |
66.11±2.37 |
61.58±1.25 |
62.81±7.79 |
68.80±6.93 |
|
His |
31.90±4.72ab |
25.09±7.33b |
28.06±14.13 |
40.79±5.43ab |
|
Lys |
78.58±4.31ab |
71.44±2.54b |
74.47±8.35 |
82.45±1.20a |
|
Arg |
79.15±2.86ab |
75.18±3.10b |
75.80±5.99 |
80.68±3.36ab |
|
Pro |
67.76±0.73b |
70.57±1.32ab |
69.36±7.39 |
68.06±2.90b |
|
TAA |
62.69±0.43ab |
59.17±1.39b |
60.83±5.69 |
66.45±3.73a |
Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.
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