MES-NA CAS NO.71119 23 8
MES sodium salt is one of the good's buffers that can be used to use systems containing metal ions, because it forms weak bonds with Ca, Mg and Mn, and its binding with Cu (II) is negligible. It is suitable for electrophoresis, tissue culture medium and other molecular biological applications.
Mes-na Buffer Product Description
MES sodium salt is the zwitterionic salt of MES in good's buffer. MES sodium salt is easily soluble in water and contains medium PKA and effective pH of 5.5-6.5. It has chemical stability and has the lowest absorption in the range of ultraviolet and visible spectra. It can be used as a buffer for pH regulation in biochemical experiments.
Detailed Introduction of Mes-na Buffer
The full name of MES sodium salt is morpholine ethanesulfonic acid sodium salt, CAS No. 71119-23-8, and the appearance property of the product is pure white powder,
It is a buffer for Biochemistry and molecular biology, suitable for the pH range of 5.5 – 6.7. PKa value is 5.9-6.3 at 25 ℃. MES sodium salt can be mixed with MES free acid or MES free acid monohydrate to obtain the required pH value.
Product Advantages of Mes-na Buffer
PKa value does not change under the influence of temperature;
The powder has good water solubility and is not easily soluble in other solvents;
There is no obvious light absorption in ultraviolet and visible light;
High purity, colorless, transparent and clear liquid after dissolution.
Advantages of Desheng Company in Mes-na Buffer
It has a professional R & D and production team of MES sodium salt, and the product parameters are stable;
At present, the purity of MES sodium salt is 99%;
The manufacturer integrating production, R & D and sales is trustworthy;
Professional receptionists are equipped before, during and after sales to protect your price;
Spot supply, large quantity, more discount.
Application of Mes-na Buffer
Commonly used in cell culture medium;
Running buffer for electrophoresis;
Protein purification in chromatography;
Buffer used to adjust pH value in other biochemical experiments.
Usage Method of mes-na Buffer
Weigh 108.61 g of MES sodium salt in 750 mL of dh2o;
Using concentrated HCl to adjust to the required pH value, this method will produce NaCl in 0.12m-0.38m concentrated stock solution;
Fill with dh2o until the final volume is 1 L;
Filtration sterilization (recommended) or high-pressure sterilization;
Store below 4 ° C.
Note: equal molar concentration of MES sodium salt and MES free acid can be mixed to a pH value of about 6.1. The pH can be adjusted by increasing the molar ratio of MES free acid
Mes-na Buffer Precautions
The unused powder shall be stored away from light, dry and sealed;
It is equipped and used now, and the use effect is better;
The containers used during configuration shall be cleaned more than 3 times to avoid exchange and pollution with other substances;
Contact or inhalation is harmful. Wear safety protection articles when using.
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