Chromatography System
Hanbon advanced chromatography systems has reliable performance and flexible configuration, and can provide Bio-Lab® laboratory chromatography system, Bio-Pro® pilot&process chromatography system, etc.
ommon Principles Of Process Scale-Up: Linear Scale-Up
Linear scale-up is the most common principle used in process scale-up. By linear scale-up, we mean that we can obtain almost uniform bioprocess chromatography results from laboratory scale to pilot results or industrial production level. The core idea is to keep the column height and linear flow rate constant and only scale up the column diameter and volume flow rate. A fixed column height and linear flow rate theoretically ensures the same retention time, the number of CVs used in chromatography and the number of CVs of the eluted sample, maximizing the same purification results. Theoretically, this linear scale-up of the purification separation behavior should be very similar, thus ensuring a successful scale-up of the ion chromatography process. However, some additional factors need to be considered, including a decrease in column tube support (increased pressure drop), column efficiency/column bed instability, sample diffusion (peak spreading), and dead volume of the system and tubing.
Conversion Of Linear Flow Rates (Cm/H) To Volumetric (Ml/Min) Flow Rates
When comparing results from columns of different sizes, it is convenient to express the flow rate as a linear flow rate (cm/h). However, flow rates are often measured as volumetric flow rates (ml/min). In order to convert between linear and volumetric flow rates, one of the following equations can be used .
Linear flow rate (cm/h) to volumetric (ml/min) flow rate
Volumetric flow rate (ml/min) = linear flow rate (cm/h) / 60 x column cross-sectional area (cm2)
=Y/60×π×d2/4
Y = linear flow rate (cm/h)
d = internal diameter of the column (cm)
e.g.:
There is a glass column (inner diameter 1.6 cm), when the linear flow rate is 150 cm/h, what is the volumetric flow rate?
Y=Linear flow rate = 150cm/h
d = inner diameter of the column = 1.6 cm
Volumetric flow rate = 150 x π x 1.6 x 1.6/(60 x 4)
From volumetric flow rate (ml/min) to linear flow rate (cm/h)
Linear flow rate (cm/h) = volumetric flow rate (ml/min) x 60/column cross-sectional area (cm2)
=Z×60×4/π×d2
Z = volumetric flow rate of the column (ml/min)
d = inner diameter of the column (cm)
e.g.:
There is a glass column (inner diameter 0.5 cm), when the volumetric flow rate is 1 ml/min, what is the linear flow rate?
Z = volumetric flow rate of the column = 1 ml/min
d = inner diameter of the column = 0.5 cm
Linear flow rate = 1 x 60 x 4/(π x 0.5 x 0.5) cm/h = 305.6 cm/h
We provide chromatography step by step method, chromatography purification method, chromatography process example, anion exchange chromatography elution, equipment used for chromatography, etc. For more information, please feel free to contact us!
Persona a contactar : com hanbon, 86 0517 83706900
Buen trato: comprar del vendedor
Por favor, lea nuestras condiciones de uso. También puede visitar nuestras preguntas frecuentes y ver nuestra información sobre los riesgos relacionados con la falsificación.
Esta página es acerca de los importadores y exportadores de Chromatography System Buscar en la categoria : B2B Servicios Buscar en la categoria : chromatography, system |
Miércoles 16 enero 2013
Cantidad : 100 - Precio : USD5159
Engine Type 449cc, 4-Stroke Single, Liquid Cooled w/Fan, Titanium 5-Valve DOHC Bore x Stroke 95mm x 63.4mm Compression Ratio 11.6: 1 Fuel Delivery Yamaha Fuel Injection (YFI), 42mm Ignition Digital TCI: Transistor Controlled Ignition Starting System Electric Transmission 5-Speed...
KRAMBA TRANSPORTATION
- 29431 - Batam
- 6285762075388
Jueves 28 mayo 2015
Have you got frustrated by spending much money in your printers or ink cartridges? Get away from all as we are here to fulfill all your printing needs. We, at angryinks, offer a best and cost effective range of Okidata toner cartridges for all the Okidata printer models. From our...
Angry Inks
- 91706 - baldwin park
- 8883232727
Lunes 23 noviembre 2015
We are one of the A-grade exporter & buying agent of block frozen shrimps/prawns of various grade/count/sizes and processed in all popular forms of the following products from Bangladesh. Our packers processing shrimp under the HACCP (Hazard Analysis and Critical Control Point)...
Northern Vegetable & Meat
- 6700 - Sirajgong
- 8801753298167
