Enzyme-linked immune test steps
Cantidad : MOQ:1box Precio : Negotiable
Enzyme-linked immune test steps
Step 1: Number: determine the number of well (samples and standards) to be used and store unused wells in 4 ℃. Every sample and standard must be parallel well (2well), and record their location
Step 2: Addtion reaction: Add 50μl standard or sample into marked well, then add 50μl HRP-conjugate solution into each well, next, add 50μl antibody working solution into each well
Step 3: Incubate: Cover with the adhesive Membrane, oscillate gently for 5s and incubate for 30 min at 25℃.
Step 4: Washing: Uncover the adhesive Membrane, discard liquid, pipette 250μl washing buffer to every well, still for 30s then drain, repeat 5 times, Pat dry with a blotting paper
Step 5: Color: Pipette 50ul Substrate A Solution, then pipette 50ul Substrate B Solution to each well, oscillate gently for 5s, avoid the light preservation for 15 min at 25℃
Step 6: Stop the reaction: Pipette Stop Solution 50μl to each well, oscillate gently, stop the reaction (the blue change to yellow).
Step 7: Calculate: Read absorbance at 450nm with microplate reader (Recommend reading the OD value at the dual-wavelength 450/630nm).finish this step within 10min.
Persona a contactar : grace xu, +13 7 94 46 93 45
Buen trato: comprar del vendedor
Por favor, lea nuestras condiciones de uso. También puede visitar nuestras preguntas frecuentes y ver nuestra información sobre los riesgos relacionados con la falsificación.
|
Esta página es acerca de los importadores y exportadores de Enzyme-linked immune test steps Buscar en la categoria : Medicina Buscar en la categoria : test, enzyme, immune, linked, steps |
Friday 06 February 2026
Palets de devoluciones Action para mayoristas, liquidación profesional y exporta
Cantidad : 30000 - Precio : 0,50 €
Ofrecemos palets de devoluciones compuestos exclusivamente por artículos procedentes de la cadena Action, dirigidos a mayoristas, profesionales del liquidación, revendedores y exportadores. Estos palets de liquidación contienen productos de gran consumo no alimentarios, seleccionados por su alto potencial de reventa y rápida rotación. Detalles de la oferta: - Palets de devoluciones de gran distribución – marca Action - Aprox. 1.000 artículos por palet (80 % de los palets) - Precio medio...
TRANSIDI
- 22440 - PLOUFRAGAN
- 09 72 63 03 26
- 33(0)603670505
Friday 05 January 2024
We are one of leading Chinese companies that build prototypes for a various industries and are advert at producing CNC machining medical parts. Using our state-of-the-art 3-axis, 4-axis and 5-axis CNC machining centers, we provide you with a wide range of CNC Prototyping Service...
YS RAPID MANUFACTURING LIMITED
- ysrapid
- 518106 - shenzhen
- 8615817251032
Saturday 19 August 2017
Symbole MC9090 Digitizer Touch écran Spécifications : Condition: New Compatible Model: Symbol MC9090 Part No.: 21-61358-01 Feature: OEM Compatible, Anti-reflective Technical principle: traditional four-wire resistive touch panel Size: 3.7 inch Material: PMMA Interface mode: plug-in...
IBarcode Solutions
- 000000 - Hong Kong
- +8613695223305






