Sample Preparation Service

The proteome comprises gene products expressed at varying levels. Preparing protein samples is a critical step in proteomics research because the quality and reproducibility of protein extraction significantly affect downstream mass spectrometry identification efficiency and accuracy. Identifying and quantitatively comparing low-abundance proteins presents a significant challenge in proteomics.

The proteome is a highly complex system. Unlike the chemically homogeneous nature of DNA and RNA, proteins exhibit considerable heterogeneity, varying greatly in size, charge, hydrophobicity, and structure. High-abundance proteins, such as actin, tubulin, or human serum albumin, along with complement system proteins and antibodies, form essential components of the proteome. With appropriate pretreatment, the low-abundance regulatory proteins most relevant to cellular responses can be more effectively detected. Moreover, many proteins undergo post-translational modifications and contain various non-peptide groups, making protein separation highly challenging. Currently, there is no universal method for protein sample preparation in proteomics research. Preparation protocols differ based on sample origin, the physical properties and subcellular localization of the target proteins, protein abundance, and matrix effects.

MtoZ Biolabs provides sample preparation services and continuously develops customized methods to meet diverse client requirements.

Altelaar A. F. M. et al. Nat Rev Genet. 2013.

Figure 1. Protein Sample Preparation Workflow

Services at Mtoz Biolabs

1. Tissue and Cell Homogenization

2. Sample Purification (Removal of DNA, RNA, and Reduction of Disulfide Bonds)

3. Removal of High-abundance Proteins

4. Protein Extraction

5. Protein Purification and Enrichment

6. Protein Digestion and Hydrolysis

Service Advantages

1. State-of-the-art Ultrafiltration Technology Platform

2. Experienced Technicians Skilled in Processing Various Sample Types

3. Comprehensive Quality Control Workflow

4. Project Design Tailored to Target Protein Characteristics

About Us
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Sample Preparation Service