Solutions For High Pressure And Baseline Drift In HPLC Columns
Remove the column outlet end
If the pressure does not drop, the HPLC column is blocked. Treatment: if the buffer salt is blocked, flush 95% water to normal pressure. If some strongly retained substances cause blockage, the mobile phase stronger than the current one should be used to rush to normal pressure. If the washing pressure does not drop for a long time according to the above method, the inlet and outlet of the column can be connected to the instrument in turn, and the column can be washed with mobile phase. At this time, if the column pressure still does not drop, only change the column inlet sieve plate, but once the operation is not very good, it is easy to cause column efficiency drop, so try to useless.
Solutions for baseline drift in HPLC columns
Generally speaking, when the machine is just started, the baseline is easy to drift, and the equilibration time is about half an hour. If you use a buffer solution or buffer salt, the equilibration time is relatively long at low wavelengths (220nm).
olutions For High Pressure And Baseline Drift In HPLC Columns
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