Specification Of HPLC Column
Hawach HPLC column is composed of a column tube, pressure cap, clamping sleeve (sealing ring), the screen plate (filter), joint, screw and so on. The column tube is made of stainless steel. When the pressure is not more than 70 kg/cm2, thick-wall glass or quartz tube can also be used. The inner wall of the tube requires a high degree of finish.
Whether filled or purchased by yourself, the performance of the HPLC column should be examined before use, and it should be re-examined during use or after placing it for a period of time. The column performance index includes column pressure under certain experimental conditions (sample, mobile phase, flow rate, temperature), theoretical tray height and tray number, symmetry factor, capacity factor, and selectivity factor repeatability, or separation degree. Generally speaking, the reproducibility of the volume factor and selectivity factor is less than ±5% or ±10%. When comparing the column effect, you should also pay attention to whether the out-of-column effect has changed.
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