Liquid Chromogenic Culture Media

Performance Characteristics of Liquid Chromogenic Culture Media

1. It is included in the "Japan Food Hygiene Guide" (2004 edition) with strong authoritative influence.

2. There are two kinds of chromogenic substances, which can directly recognize coliforms and E. coli.

3. The test results can be obtained within 24 hours, but BGLB needs 48 hours.

4. No need for a fermentation tube and a constant temperature pool with low cost and easy operation.

5. No need to prepare medium and wash tubes (except XM powder medium).

6. No need for identification and supplementation experiments such as EMB culture.

7. E.coli O157 cannot be identified separately but qualitative testing only.

Product Details of Liquid Chromogenic Culture Media

Usage Method of Liquid Chromogenic Culture Media

1. Container surface sterilization: The surface of the aluminum foil should be disinfected with an lamp before use.

2. Opening: Open the aluminum foil with an MT opener (OS-01) or open directly with a sterile pipette.

3. Inject the sample diluent: use a pipette to inject the sample dilution into the cup

XM-30: 1ml, XM-31: 10ml, XM-32: 5ml

4. Cultivation: Culture time: 24 hours; Culture temperature: 36 ± 1 °C

5. Disposal: Dispose of them after sterilization

※XM powder medium usage: Dissolve 500ml of pure water per bag, inject 10ml into the test tube, autoclave, then inject 1ml sample, and incubate at 36±1°C for 24 hours. The identification method is the same.

Coloring Principle

Coliforms positive

There is a special β-galactosidase only exit in the coliforms. It can be used as an indicator enzyme for coliform bacteria, which catalyzes the hydrolysis of the substrate X-GAL to form 5,5-dibromo -4,4-dichloro indigo, appearing light blue ~ purple-blue.

E. coli positive

β--glucuronidase can be used as an indicator enzyme for Escherichia coli; because β-glucuronidase can be observed in Escherichia coli, Salmonella and Shigella in intestinal bacteria, and 95% of E. coli contains β-glucuronidase. It catalyzes the hydrolysis of MUG to form 4-methylumbelliferon, which exhibits fluorescence at 366 nm ultraviolet light and is visually discernible under indoor light intensity.

In the case of neutralization of disinfectants such as quaternary ammonium is quite important to the antimicrobial activity, it is recommended to use Letheen Broth of Swab Sampler to test preservative materials.